Glutamic acid-insensitive [3H]kainic acid binding in goldfish brain

Glutamic acid-insensitive [3H]kainic acid binding in goldfish brain.

Kainic acid is supposed to be a specific agonist for a subclass of excitatory glutamate receptors in the vertebrate CNS. An investigation of (2 nM) [3H]kainic acid binding sites in goldfish brain, using quantitative autoradiography, has revealed evidence for two types of kainic acid receptors which differ in sensitivity to glutamic acid. L-Glutamic acid (0.1-1 mM) displaced over 95% of specific...

Glutamic acid metabolism in perfused cat brain studied with 14C-labelled glutamic acid.

The rate of transport of blood glutamic acid into the brain and the rate of metabolic conversion of the amino acid in the brain were derived by the use of the brain perfution method in vivo and in situ with [D.HC] ·Lglutamic acid. The net uptake of glutamic acid by the brain was observed. Most of the radioactivity released from the brain into the cerebral venous blood was found to consist of gl...

Accumulation of glutamic acid in isolated brain tissue.

Van Slyke (1913) was the first to show that most aniimal tissues have a far higher concentration offree amino-acids than has blood plasma. For example, 75, 73 and 64 mg. non-protein amino N were found in 100 g. ofdog liver, kidney and spleen respectively, and only about 4-5 mg. in 100 g. of blood plasma (Van Slyke & Meyer, 1913). Hamilton (1945) and Krebs, Eggleston & Hems (1949) have reported ...

Determination of Glutamic Acid

Glutamic Acid Dehydrogenase and Glutamic-oxalacetic

As part of a larger project dealing with an in vestigation of amino acid and protein metabolism in leukemia, a study was undertaken to determine those enzymes concerned with amino acid anabo lism and catabolism as they occur in selected tis sues. Several review papers (@,4, 11, 1@)have ade quately summarized our present knowledge of the metabolism of white blood cells (WBC) in leuke mia. Howeve...